AgNORs计数与肝硬化、增生结节和肝癌的关系

RELATIONSHIP BETWEEN AGNORS COUNTS AND LIVER CIRRHOSIS, HYPERPLASTIC NODULES AND HEPATOCELLULAR CARCINOMA

用核仁组成区相关嗜银蛋白(AgNORs)染色技术对肝硬化、增生结节和肝癌进行了AgNORs定量测定.结果表明,正常肝组织的AgNORs计数为1.48±0.63,肝硬化为1.73±0.75,嗜酸性细胞结节为2.44±1.08,Ⅰ级肝癌为2.77±1.22,Ⅱ级肝癌为4.37±1.64,Ⅲ级肝癌为5.57±2.10;嗜酸性细胞结节属活跃增殖性病变,其AgNORs计数与正常肝组织有明显差异(P<0.05),与Ⅰ级肝癌相近(P>0.05).提示AgNORs计数与肝癌分级有一定关系,嗜酸性细胞结节为癌前期病变且与肝硬化结节为两种不同的病变.

The nucleolar organizer regions associated proteins (AgNORs) in liver cirrhosis, hyperplastic nodules and hepatocellular carcinoma(HCC) were quantatively studied by means of the argyrophil technique. The results showed that the AgNORs counts were 1.48±0.63 in the normal liver, 1.73±0.75 in liver cirrhosis, 2.44±1.08 in acidophilic hyperplastic nodules, 2.77±1.22 in HCC grade Ⅰ , 4.37±1.64 in HCC grade Ⅱ , 5.57±2.10 in HCC grade Ⅲ. In acidophilic hyperplastic nodules, which belong among actively proliferating lesions, the AgNORs counts were significantly higher than in the normal liver (P<0.05) and approached those in HCC grade Ⅰ (P>0.05). These results reveal that AgNORs counts are related to the grading of HCC. Acidophilic hyperplastic nodules which are preneoplastic lesions of HCC, are a different kind of lesion from liver cirrhosis.