p21ras和Cyclin D1在肺癌组织中的表达及相互关系

Expression of p21ras and Cyclin D1 and their relationships in human lung cancer tissues

目的 研究p2 1ras、CyclinD1在肺癌组织中的表达及其相互关系。方法 运用免疫组化S -P法检测p2 1ras、CyclinD1蛋白在 6 8例肺癌组织中表达情况。结果 p2 1ras在肺腺癌、肺鳞癌及小细胞肺癌中阳性表达率分别为 96 .6 7%、80 .6 5 %及 4 2 .86 % ,三者之间差异具有显著性 (P <0 .0 5 ) ;直径≤ 3cm的肿瘤p2 1ras全部为阳性表达 ,而直径 >3cm的肿瘤p2 1ras的阳性表达率为 83.82 % ,二者之间的差异具有显著性 (P =0 .0 5 ) ;p2 1ras在分化较好的肿瘤 (Ⅰ +Ⅱ级 )和分化较差的肿瘤 (Ⅲ级 )的阳性表达率分别为 89.80 %、6 8.4 2 % ,二者之间差异有显著性 (P <0 .0 5 )。CyclinD1在肺腺癌、肺鳞癌及小细胞肺癌中阳性表达率分别为 5 0 .0 0 %、4 1.94 %及 2 8.5 7% ,三者之间阳性表达率无显著性差异 (P >0 .0 5 ) ;CyclinD1表达水平与临床病理分期、淋巴结转移相关 (P <0 .0 5 ) ,而与其他临床病理因素无关。p2 1ras和CyclinD1表达无明显相关性 (P >0 .0 5 )。结论 p2 1ras表达可能与肺癌的组织学类型、分化程度有关 ;肺癌组织中CyclinD1表达可能与肿瘤淋巴结转移有关。

Objective To study the expression of p21ras and Cyclin D1 and their relationships in human lung cancer tissues. Methods Immunohistochemical S-P method was used to examine the expression of p21ras and Cyclin D1 in 68 cases of lung cancer. Results The positive rate of p21ras was 96.67%, 80.65% and 42.86% in adenocarcinoma, squamous carcinoma and small cell lung cancer (SCLC) respectively, showing significant difference (P<0.05). The positive rate of p21ras was 100% in the cancers ≤3 cm in diameter, but was 83.82% in the bigger ones with diameter >3 cm (P≤0.05). Also, it was higher in the better differentiated cancers (gradesⅠand Ⅱ) than in the poorly differentiated grade Ⅲ cancers (92.6% vs. 68.42%, P<0.05). The positive rate of Cyclin D1 was 50%, 41.94% and 28.57% in adenocarcinoma, squamous carcinoma and SCLC respectively, showing no significant difference (P>0.05). The expression of Cyclin D1 was associated with TNM stage and lymph node metastasis(P<0.05), but not correlated to other clinico-pathological factors. The expressions of p21ras and Cyclin D1 were not correlated(P>0.05). Conclusion The expression of p21ras is related to the histological type and degree of differentiation of the lung cancer. The expression of Cyclin D1 in lung cancer may be associated with its lymph node metastasis.