T-2毒素抗原的制备与ELISA法检定

Preparation of T-2 toxin antigen and determination by ELISA

目的 制备 T- 2毒素抗原 ,为建立检测 T- 2毒素的 EL ISA法奠定基础。方法 T- 2毒素与琥珀酸酐在吡啶参与下于蒸汽浴中反应 ,生成 T- 2 -半琥珀酸酐后 ,再与小牛血清白蛋白经碳二亚胺偶联结合生成 T- 2毒素抗原。结果 利用琥珀酸酐法成功制备 T- 2毒素抗原 ,即 BSA- T- 2 HS。结论 T- 2毒素抗原的制备为 EL

Objective To prepare T 2 toxin antigen so as to establish ELISA for detecting T 2 toxin.Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS.At the presence of EDPC, T 2HS conjugated with BSA,then BSA T 2HS was prepared.Results T 2 toxin antigen was prepared by the method of Succinic Anhydride.Conclusions Preparation of T 2 toxin laid a foundation for ELISA.