摘要
采用RT-PCR方法成功地从人胎盘组织扩增出hIGF- 基因。将其连入表达载体pET30a(+)质粒中,SDS-PAGE结果证明,pET30a(+)-hIGF- 在大肠杆菌BL21(DE3)中获得高效表达,目的蛋白占总菌体蛋白35%左右。
Human IGFⅡ gene was successfully amplified from human placenta with RTPCR method,then cloned it into expression vector:pET30a(+).pET30a(+)hIGFⅡ was highly expressed in BL21(DE3) and the recombinant hIGFⅡ was about 35% of total proteins measured with SDSPAGE.
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2003年第5期133-135,共3页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家"863"计划项目(2001AA213081)
西北农林科技大学重点专题基金项目