应用间接血凝(IHA)诊断绵羊肉孢子虫病

DIAGNOSIS OF SHEEP SARCOSPORIDIOSIS USING INDIRECT HEMAGGLUTINATION

选用自然感染严重的绵羊肉孢子虫病病羊肌肉和从食道挑出的虫体制备诊断抗原,用微量间接血凝法对326只绵羊血清进行诊断。结果表明,34只(份)带虫绵羊血清检出阳性反应32只(份),阳性符合率达94%,血凝效价达1∶64—1∶1024。9只(份)健康羊血清均为阴性反应,血凝效价仅达1∶4—1∶16。240只(份)被检0.5岁羔羊血清中有166只(份)血清呈阳性反应,阳性率达69.2%。被检成年羊血清43只(份),41只(份)血清呈阳性反应,阳性率达95.33%。试验表明,用绵羊肉孢子虫滋养体制作的可溶性蛋白抗原(蛋白含量为200—300μg/ml),致敏健康绵羊红细胞作为诊断液,敏感性高,重复性稳定,特异性强,不与弓形虫和棘球蚴发生交叉反应。是一种较为理想的诊断方法。

Diagnostic antigen was prepared from the worms picked out from the se-verely natural infected sheep with Sarcocystis and 326 sheep sera were ex-amined by the indirect mierohemagglutination test.The results showed thatout of 34 sere of the infected sheep with Sarcocystis,32(94%)were posi-tire,the hemagglutination titers were 1:64—1:1024.The 9 sera fromhealthy sheep were all negative,the serum titres only 1:4—1:16.of 240sera of 6-month-old lambs,166 (69.2%) were positive,of 43 sere of exam-ined adult sheep,41 (95.33%) were positive.The experiment showed thatthe use of the diagnostic liquid,which was made from the soluble proteinantigen (protein contents range 200—300 ug/ml)prepared from sheep Sarco-cysity sporadin sensitizing the red blood cells of healthy sheep was an ide-al diagnostic method with high sensitivity,stable repeatability,strong spec-ificity and no cross reaction with toxoplasmosis and echinococcosis.