缺血预处理对心肌组织酶的活性影响实验研究

Effect of Ischemic Preconditioning on Activities of Phospholipase A2 and ATPase of Myocardial Cells during Cardiopulmonary Bypass

目的 观察缺血预处理 (IPC)对体外循环 (CPB)中缺血再灌注心肌组织磷酯酶A2 (PLA2 )和ATPase活性的影响 ,评价其对心肌的保护作用 ,并初步探讨其可能的作用机制。方法 在猫CPB模型的基础上 ,比较IPC组和缺血再灌注组CPB中心肌组织PLA2 及Na+ -K+ -ATPase、Ca2 + -Mg2 + -ATPase、Ca2 + -ATPase活性。结果 IPC处理组可明显减轻CPB中缺血再灌注导致的PLA2 活性升高和ATPase活性下降。结论 IPC可能通过减轻缺血再灌注期间的Ca2 +

OBJECTIVE To elucidate the influences of ischemic preconditioning(IPC)on activity of Phospholipase A 2 and ATPase of ischemia / reperfusion myocardial cell during cardiopulmonary bypass(CPB).METHODS Ninety felines were randomized into there groups: GroupⅠ(n=30),in which CPB was conducted without aortic cross-clamping (ACC); Group Ⅱ(n=30),with 60min ACC followed by 90 min reperfusion, and cardioplegia used during period of ACC; Group Ⅲ(n=30), with protocol similar to that of group Ⅱ except for three-round 15 min IPC applied before ACC. Activities of PLA 2 and Na +-K- +ATPase、Ca 2+-Mg 2+-ATPase andCa 2+-ATPase of myocardial cells were simultaneously measured during ACC and reperfusion periods. RESULTS IPC significantly alleviated the upregulation of activity of PLA 2 and the inhibition of activity of Na +-K- +ATPase、Ca 2+-Mg 2+-ATPase and Ca 2+-ATPase of myocardium during periods of ischemia and reperfusion. CONCLUSION IPC can protect myocardial cells from ischemia and reperfusion injury during CPB by regulating the activities of PLA 2 and the ATPases of myocardium, which may alleviate of Ca 2+overload and hydrolysis of membrane phospholipid.