摘要
本文以苯甲酸为内标,采用高效液相色谱分析电解硝基苯产物中对氨基酚及硝基苯的含量。紫外检测器检测波长254nm,色谱柱为μ-BondapakC18柱,流动相组成为0.05mol/L磷酸盐缓冲液(pH3.5)+甲醇(70+30,V/V)。实验求得对氨基酚,硝基苯的回归方程分别为y=12.75x+0.60和y=37.46x+3.71。其相关系数分别为0.9995及0.9981。上述待测组分对内标物苯甲酸的相对校正因子分别为2.42和0.162。
The contents of paminophenol and nitrobenzene in the electrolytic solution were determined by HPLC with μBondapak C18 column,UV detection at 254 nm, and benzoic acid as internal standard. The mobile phase was made up of methanol+0.05 mol/L(pH3.5)monopotassium phosphate buffer solution (30+70,V/V).The regression equation of paminophenol, benzoic acid and nitrobenzene were y=12.75x+0.60, y=19.94x-1.69 and y=37.46x+3.71, respectively. Their correlation coefficient were 0.9995, 0.9983 and 0.9981.Their relative corrective factor to the internal standard substance were 2.42, 0.162.
出处
《分析科学学报》
CAS
CSCD
北大核心
2003年第4期349-351,共3页
Journal of Analytical Science
基金
广西自治区科学基金(No.0007003)
关键词
对氨基酚溶液
高效液相色谱
内标法
苯甲酸
硝基苯
pAminophenol
High Performance Liquid Chromatography
Internal standard method