反相高效液相色谱测定雷公藤浸膏中甲素的含量

RP-HPLC Determination of Triptolide Content in Tripergium Wilfordii Herbal Pieces Solid

报道了液固萃取-反相高效液相色谱法测定雷公藤浸膏(乙酸乙酯提取物)中甲素含量的方法。用LichrospherC18色谱柱,以甲醇∶水=60∶40(V/V)溶液为流动相,检测波长218nm。方法回收率为99.4%,变异系数为0.53%,方法快速,重现性及准确度能满足雷公藤浸膏中甲素含量测定的要求。

The liquidsolid extractionRPHPLC method for the determination of triptolide in commercial Tripergium Wilfordii Herbal extraction has been reported. The sample was separated on Lichrospher C18(25 cm×4.6 mm i.d., 5 μm) column with mobile phase of methanol∶water=60∶40(V/V). The detection was performed by using the photodiode array detector at 218 nm.The recovery was 99.4% with a CV of 053%. This method was quick, accurate and reproducible, and can be used for monitoring the quality of Tripergium wilfordii Herbal pieces solid in the market.