摘要
在未涂层的石英毛细管中,以0 04mol/LNa2HPO4 0 04mol/LNaH2PO4的混合体系(pH7 32)为运行缓冲液,采用紫外检测波长为200nm,建立了果蔬中谷胱甘肽(GSH)的毛细管电泳测定方法。结果表明:GSH为3 07~307mg/L时具有良好的线性关系,相关系数r=0 9995;最低检测质量浓度为1 54mg/L(S/N=3)。利用该方法对黄瓜及西红柿中的GSH进行了测定,在3个添加水平上进行加标回收试验,结果GSH在西红柿中的加标回收率分别为99 04%,94 96%和101 4%,相对标准偏差(RSD)分别为1 53%,3 78%和1 89%;GSH在黄瓜中的加标回收率分别为91 25%,94 82%和99 35%,RSD分别为1 46%,3 47%和4 00%。该方法具有快速、简便、灵敏、重现性好等特点。
A method for the determination of glutathione (GSH) in tomatoes and cucumbers by capillary electrophoresis with UV detection (200 nm) was developed. After simple treatment of the samples, separation and determination of GSH was obtained within 7 min by using the phosphate buffer system containing 004 mol/L Na2HPO4004 mol/L NaH2PO4 (pH 732). The quantitative analysis was performed with external standard under the optimal conditions. The calibration curve of GSH showed good linearity in the range of 307-307 mg/L with r=0999?5, and the detection limit was 154 mg/L (S/N=3). For spiked tomato samples with standard at three levels (9219, 1229 and 1537 mg/L), the recoveries were 9904%, 9496% and 1014%, and the RSDs were 153%, 378% and 189%, respectively. For cucumbers, the recoveries were 9125%, 9482% and 9935%, and the RSDs were 146%, 347% and 400%, respectively. The method has the advantages of being simple, convenient and sensitive. The method can be used for the routine analysis of GSH in fruits and vegetables.
出处
《色谱》
CAS
CSCD
北大核心
2003年第5期510-512,共3页
Chinese Journal of Chromatography
基金
福建省自然科学基金资助项目(批准号:C0110007).