中国狼的种内系统发育关系与亚种分化

The Phylogenetic Relationship and Subspecies Differentiation of Chinese Wolf

通过对来自 5个地区、11个亚地区的 4 3个中国狼 (Canislupuschanco)样品的mtDNA分析 ,得到了 14个单倍类型。应用Mega 2 .1计算机软件包构建了NJ树。系统树的拓扑图显示 ,中国狼的母系群体来源于两个世系 ,且世系 1要比世系 2古老。此外 ,无论是在亚地区还是在地区的群体间 ,都存在明显的基因交流 ,使得地区间和地区内群体的遗传分化不明显。但是 ,结合序列分析、拓扑结构图和遗传距离分析认为 ,青藏和西南群体与我国其它地区群体具有等同的分类地位。

The mtDNA of the forty-threes samples of Chinese wolf (Canis lupus chanco) in eleven sub-regions belonging to five regions, was studied. Fourteen haplotypes were found. NJ tree was constructed by Mega 2.1 software package. The topology of molecular phylogenetic tree showed that there were two lineages in the maternal populations of Chinese wolf, and the lineage 1 was older than the lineage 2. The genetic divergence between sub-regional or regional populations was not distinct because of the gene flow. The same classification status existed in the population of Qingzang altiplano and Southwest region as well as other regional populations, judging from the synthetic analysis of sequence variance, topological structure and genetic distance among regional populations.