基于RAPD分析用的佛手DNA的提取

DNA extraction for RAPD analysis in Bergamot

对佛手DNA提取过程从不同方面进行了改良、优化 ,筛选出较理想的佛手DNA提取方法 :每eppen dorf管鲜样品取量为 0 .1g～ 0 .2 5 g ,70 0 μL提取缓冲液 ,加提取液前用液氮研磨 ,Vc(抗坏血酸 )添加量为w(VC/鲜样 ) =0 .1.该方法分离出DNA的A2 60 /A2 80 大于 1.9,符合佛手RAPD(随机扩增多态DNA)分析要求 ,在佛手遗传多态性分析和品种分子鉴定中具有良好的应用前景 .

It was improved and optimized by the authors the course of bergamot DNA extraction from different aspects,filtrated an ideal bergamot DNA extraction method [0.1 g～0.25 g fresh sample,700 μL extraction buffer, liquid nitrogen whet,w(Vc/ fresh sample)=0.1].The A-{260}/A-{280} values of isolated DNA was above 1.9. This method coincided with the requisition of RAPD analysis, and was promising for bergamot genetic polymorphism analysis and cultivar identification.