滩羊体大品系遗传标记的研究

Research on the hereditary marker of bigger strain of Tan sheep

采用RAPD(Rondom Amplified Polymorphic DNA)技术,利用混合基因池(DNA pool)法,对滩羊体大品系、普通品系进行了DNA多态性分析,从100种具有10个碱基的随机引物中,筛选出84种引物在滩羊群体基因组中共扩增出358条带,其中22种引物的扩增产物表现为多态(占22％),且扩增出32条有差异的条带,占总带数的8.94％;62种引物的扩增产物表现为单态(占62％)。滩羊体大品系的特异性条带有5条,而普通品系的特异性条带有7条,这些特异标记可以用来鉴定滩羊的两个品系;滩羊体大品系与普通品系间的遗传距离为0.136±0.087,表明两品系之间的亲缘关系很近。

Rrandom amplified polymorphic DNA (RAPD) was used to screen the DNA fingerprints about two strains: the bigger strain and the ordinary strain of Tan sheep, 84 polymorphic primers out of 100 10 bp random primer were amplified in genome DNA of Tan sheep, total of 358 bands from these polymorphic primers were scored, and 32 different bands (8.94%) amplified from 22 primers were polymorphic, five of the special marked were found in bigger strain of Tan sheep, while seven of them were found in ordinary one, these can be used to identify two strains of Tan sheep. The genetic distance between the bigger strain and the ordinary strain of Tan sheep is 0.136 + 0.087. The results indicated that phylogenetic relationship is closer between two strains of Tan sheep.