新疆甜菜坏死黄脉病毒RNA5的检测及核苷酸序列分析

Detection and Nucleotide Sequence Analysis of Beet Necrotic Yellow Vein Virus RNA5 Isolated from Xingjiang

利用RT-PCR方法,对12个新疆不同地区的甜菜坏死黄脉病毒(BNYVV)分离物和美国的BN-TX及黑龙江的He分离物进行检测,结果表明,只有库尔勒地区的所有分离物和来自黑龙江的He分离物含有RNA5,其他地区的分离物未检测到RNA5。选K2和He分离物进行序列分析,测得K2分离物长为1323 nt,He分离物长为1300 nt;与国内外报道的中国包头B分离物、呼和浩特H、日本的D5分离物和法国的F72分离物的RNA5序列相比,序列同源性K2为95.0％～96.8％,He为96.0％～99.3％,均含有一个开放阅读框(ORF),由此推导出的各分离物的氨基酸同源性,K2为93.0％～96.5％,He为96.5％～99.6％。其变异主要集中在富含A的区域。

Using RT-PCR method,the RNA5 genomic component waw detected for 12 beet necrotic yellow vein virus (BNYW) isolates from different beet production areas in Xingjiang. The result showed that RNA5 was only found in all kurle isolates and Heilongjiang isolate He,but not in isolates from other areas. Nucleotide sequence were analyzed for K2 and He,the results demonstrated that the RNA5 genome of K2 and He and 1323 nt and 1300 nt in length respectively, which contained single open reading frame(ORF). Comparing with the published sequences of B,H,D5 and F72 isolates,K2 and He isolates shared 95. 0%-96. 8% and 96. 1%-99. 3% nucleotide identity respectively deduced 26 KD protein amino acid identity of K2 and He is 93. 0%-96. 5% and 96. 5%-99. 6% respectively. The variation of genome is mainly in A-rich region.