多肽片段连接方法及肽硫酯和肽醛的合成

Peptide Segments Ligation and Synthesis of Peptide Thioester and Peptide Aldehyde

本文综述了多肽和蛋白质合成中的片段连接方法 ,这是近年来多肽和蛋白质合成领域中方法学上的重要进展。该方法使用非保护的多肽片段 ,无需酶或化学活化试剂 ,在缓冲溶液中能够高产率地获得多肽和蛋白质。还介绍了与多肽片段连接有关的肽硫酯和肽醛的合成方法。

This review focuses on peptide segment ligation for synthesis of peptides and proteins. Ligation is a new methodology in peptide and protein synthesis chemistry. Peptide and protein can be obtained with high yield in aqueous buffer by coupling unprotected peptide segments without activation by enzymatic or chemical agents. The synthetic methods of peptide thioester and peptide aldehyde are also discussed.