摘要
采用响应面方法对康氏木霉(Trichodermakoningii)ZJ5生产纤维素酶的培养基进行了优化.首先通过全因子实验分析培养基组分;稻草粉、麦麸、大麦粉和(NH4)2SO4对纤维素酶三个组分活性的影响,确定主要影响因子为稻草粉和(NH4)2SO4,前者为正影响,后者为负影响,用最陡爬坡路径逼近最大响应区域.利用中心组合设计及响应面分析确定主要影响因子的最佳浓度.在优化培养基中发酵6d,CMC酶活、滤纸酶活和β-葡萄糖苷酶活分别达到了765.8、155.3U/mL和39.54U/mL.
Response surface methodology was used to optimize a medium for cellulase production of Trichoderma koningii ZJ5. In the first optimization step the effect of straw powder,barley flour,wheat bran and (NH4)2SO4 on cellulase production was evaluated using a full factorial design. Straw powder had positive effect,while (NH4)2SO4 had negative effect on cellulase production. The path of steepest ascent was used to approach the optimal region of the medium composition. The optimal concentrations of straw powder and (NH4)2SO4 were determined by a central composite design and response surface analysis.The maximum activity of carboxymethyl cellulose enzyme (CMCase),filter paper enzyme (FPA) and β- glucosidase is 765.8 U/mL,155.3 U/mL and 39.54 U/mL,respectively under the optimized culture medium after 6 days.
出处
《浙江大学学报(工学版)》
EI
CAS
CSCD
北大核心
2003年第5期623-628,共6页
Journal of Zhejiang University:Engineering Science