基于普鲁士蓝纳米立方的类过氧化物酶活性检测还原型谷胱甘肽

Prussian Blue Nanocubes(PB NCs)Peroxidase Mimetic-based Method for Glutathione Detection

本研究开发了一种基于普鲁士蓝纳米立方体(PB NCs)类过氧化物酶活性检测还原型谷胱甘肽(GSH)的方法.PB NCs具有类过氧化物酶活性,可催化过氧化氢氧化3,3',5,5'-四甲基联苯胺(TMB)生成氧化态的TMB(oxTMB),在652nm处有较强的紫外-可见吸收峰;GSH既可以与PB NCs表面Fe3+结合,又可还原oxTMB,这双重作用抑制了TMB的氧化过程,引起体系紫外-可见吸收峰强度降低.基于体系紫外-可见吸收峰强度随GSH浓度的变化关系,我们得到GSH检测线性范围为0.01-5.0μmol/L.该方法具有较高的灵敏度和较好的选择性,不仅为GSH的检测提供了一种新的思路,还拓展了PB NCs在生物分析检测中的应用.

In this paper,a novel method for glutathione detection is developed based on the peroxidaselike activity of Prussian blue nanocube(PB NCs).PB NCs catalyzes the oxidation of TMB by H2 O2 to obtain the oxidized TMB(oxTMB)with a maximum absorption at 652 nm.The introduction of glutathione can cheat with Fe3+on the surface of PB NCs and cause the reduction of oxTMB,both of which inhibit the oxidation of TMB,resulting in the decrease of the absorbance at 652 nm.Based on the relationship between the changes of absorbance and the concentration of glutathione,a linear relationship in the glutathione range from 0.01 to 5.0μmol/L is obtained.This method shows high sensitivity and good selectivity,which may not only provide a new idea for glutathione detection,but also expand the application of PB NCs in bioanalytical detection.