低剂量电离辐射对小鼠睾丸生精细胞凋亡的影响

EFFECT OF LOW DOSE X-RAY IRRADIATION ON APOPTOSIS IN SPERMATOGENIC CELLS OF MOUDE TESTES

研究不同低剂量X射线对小鼠睾丸生精细胞凋亡的影响。采用常规苏木精-T尹红(HE)染色法和原位末端标记术(TUNEL)通过光镜观察不同低剂量X射线照射后小鼠睾丸在生精周期不同阶段各类生精细胞凋亡的剂量及时程效应关系。结果表明,低剂量电离辐射诱导生精细胞凋亡具有明显的细胞种类规律性,在较低剂量照射(0.025Gy)时,以精原细胞凋亡为主,随剂量增加(0.05—0.2Gy)才逐渐累及精母细胞,并且精原细胞凋亡率明显高于精母细胞,很少累及精子细胞和精子。低剂量电离辐射诱导生精细胞凋亡具有一定规律性,这为深入探讨低剂量辐射兴奋效应机制提供了更深层次的实验证据。

To study the effects of low dose radiation (LDR) with different doses of X-rays on the apoptosis in spermatogenic cells of male Kunming mouse testes. The time-effect and dose-effect of apoptosis in the different stages of spermatogenic cell cycles of mouse testis after LDR with different doses of X-rays were studied with light microscope using the methods of TdT-mediated dUTP nick end labeling (TUNEL) and HE staining. The apoptosis of spermatogenic cells induced by LDR had a remarkable regularity in cell types. When the dose was 0.025Gy, spermatogonium apoptosis was taken as main. With the dose increase of irradiation (0.025—0.2Gy), spermatocytes also showed an apoptotic change, but the apoptotic rate of spermatogonia was significantly higher than that of spermatocytes. Moreover, the apoptosis of spermatids and spermatozoa scarcely occurred after irradiation with low dose. The apoptosis of spermatogenic cells induced by LDR has a regular change, which provides a further experimental evidence for the mechanism study of hormesis by LDR.