油菜雌性不育突变体FS-M_1的生物学特性研究

A primary study on biological characteristics of female sterile mutant FS--M_1 of rapeseed (Brassica napus L.)

对甘蓝型油菜雌性不育突变体FS-M_1的研究结果表明,FS-M_1天然授粉、人工授粉自交、自交套袋隔离、兄妹互交、剥蕾自交结角率均为3％左右,平均每角粒数1.3～1.8粒,表明雌性不育性稳定。用FS-M_1花粉授予正常油菜品种,杂交结实率正常,F_1植株自然授粉结实正常F_1植株自交结角率为97％,平均每角21粒,雌性不育花粉对F_1育性无异常影响。电镜扫描发现:FS-M_1柱头乳突细胞扁平干瘪,发育畸形,可能是形成FS-M_1雌性不育的主要原因。

Study on female sterile mutant FS--M_1 of rapeseed (Brassica napus L.) showed that pod set ratio of open--pollinated, artificial--pollinated, self--pollinated, sibling--pollinated, and self--pollinated by opening bud was about 3% , and seed number per pod was 1.3～1.8. It indicated that female sterility of FS--M_1 was steady. Number of seeds of hybrid of normal euhivar×FS--M_1 was normal, so does open--pollinated of F_1. The pods set ratio of self--pollinated of F_1 was 97% , and seeds per pod reached 21. It indicated that the FS--M_1 pol- lens didn't affect the fertility of F_1. Scanning the stigma by electrical mirror showed that papillae cell of FS--M_1 was flat and shriveling. This maybe explain why FS--M_1 was female sterility.