改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留

Determination of Four Fluoroquinolones Drug Residues in Aquatic Products by Capillary Electrophoresis-Electrochemiluminescence with Modified Gold Nanoparticles Enrichment

建立了一种利用改性纳米金粒子富集与毛细管电泳-电化学发光(CE-ECL)法测定水产品中4种氟喹诺酮(环丙沙星、恩诺沙星、氧氟沙星、诺氟沙星)类药物残留的分析方法。实验考察了富集条件与CE分离条件,并基于增强Ru(bpy)23+电化学发光的原理,优化了ECL检测条件。结果表明,在最优条件下,经改性金纳米粒子富集后的4种分析物在0. 05～10. 0μmol/L浓度范围内,其峰高与浓度呈现良好的线性关系,检出限(S/N=3)可达0. 2μmol/L,4种目标物的富集倍数达104～127倍。该方法用于鳗鱼样品的分析,回收率为94. 5%～112%,相对标准偏差(RSD)均不大于6. 3%。

A method was established for the determination of 4 fluoroquinolones,i. e. ciprofloxacin( CIP),enrofloxacin( EN),ofloxacin( OF) and norfloxacin( NOR) in aquatic products by capillary electrophoresis-electrochemical luminescence( CE-ECL) with modified gold nanoparticle enrichment. The conditions for enrichment and CE separation were studied,and the conditions for ECL detection were also investigated based on the principle of enhanced Ru( bpy)23 +electrochemiluminescence. Under the optimal conditions,there were good linearities for the 4 analytes enriched with modified gold nanoparticles in the concentration range of 0. 05-10. 0 μmol/L. The detection limits( S/N = 3) were low to 0. 02 μmol/L and the enrichment times were 104-127. This method was used in the analysis of 4 analytes in actual eels samples with recoveries of 94. 5%-112% and relative standard deviations( RSDs) not more than 6. 3%.