DNA碱基激光拉曼光谱检测方法的研究

Study on Detection of DNA by Laser Raman Spectrometry

以激光拉曼光谱仪采集腺嘌呤(A)、鸟嘌呤(G)、胞嘧啶(C)、胸腺嘧啶(T)的拉曼光谱图,分析了各碱基谱峰归属,并依据归属结果检测混合碱基组成。结果显示:4种碱基的特征峰集中在175～1 800cm^(-1)。相同积分时间和激光功率条件下,该波段内嘌呤碱基的激光拉曼光谱峰高于嘧啶碱基的谱峰。腺嘌呤、鸟嘌呤、胞嘧啶、胸腺嘧啶分别在723、648、791、1 368 cm^(-1)处的谱峰最强,利用这些特征峰能在2min内鉴别出混合碱基中的各碱基。

A method of laser Raman spectrometry was developed for the detection of DNA bases. The Raman spectra of adenine( A),guanine( G),cytosine( C) and thymine( T) were collected with laser Raman spectrometer in this work. The characteristic peaks in the spectra for each DNA base were analyzed,which were used to determine the components of DNA base mixtures. Results demonstrated that the characteristic peaks mainly ranged from 175 cm-1 to 1 800 cm-1. The peaks of A and G were higher than those of C and T when the integration time and laser power were certain. The strongest Raman signals for A,G,C,and T are at 723,648,791 and 1 368 cm-1,respectively.Each DNA base could be determined by using the characteristic peaks from the mixed DNA bases within 2 min.