反义Wx基因导入我国常规籼稻品种的研究

TRANSFORMATION OF THE ANTISENSE WAXY GENE INTO THE CONVENTIONAL INDICA RICE VARIETIES

经农杆菌介导,将自行克隆并构建的反义Wx基因导入4个高直链淀粉含量的常规籼稻品种绿黄占、清芦占11号、三芦占7号和特青中,经潮霉素抗性筛选获得抗性植株。聚合酶链反应(PCR)和Southern杂交检测证明反义Wx基因已整合进转基因水稻植株的基因组中。对成熟种子直链淀粉含量的分析表明,部分转基因水稻植株T_1或T_2代种子中的直链淀粉含量有不同程度的下降,最低的已降至16.52％,较对照下降了40.5％;在此基础上筛选获得了部分转基因纯合株系。研究结果还表明,直链淀粉含量的改变会导致相应稻米的胶稠度和糊化温度的变化。

In order to improve the quality of the conventional indica rice varieties in China, a chimeric antisense Wx construct was successfully introduced into four conventional Indica cultivars, including Luhuangzhan, Qingluzhan 11, Sanluzhan 7 and Teqing. Many independent transgenic rice lines were produced. PCR and Southern blotting analysis showed that the transgene was integrated into the genome of the transgenic rice plants. The amylose content in the endosperm of mature seeds from some T1 and T2 transgenic plants was measured, and the results showed that the amylose content in some grains reduced. The lowest was reduced to 16. 52%, which was 40. 5% lower than that of the untrans-formed grains. Based on the above results, several transgenic lines with homozygous transgene were selected, and the other factors influencing rice cooking and eating quality were also analyzed, which revealed that the reduction of the amylose content had some influence on the gel consistency and gelatinization temperature in corresponding rice grains.