灰葡萄孢对速克灵抗性的研究

RESISTANCE OF BOTRYTIS CINEREA TO PROCYMIDONE

从江苏各地采集番茄等15种蔬菜灰霉病标本,经分离获灰葡萄孢(Botrytis cinerea Pers.)127株。以速克灵有效成分5μg·mL^(-1)为标准,检测灰葡萄孢对速克灵的抗性。结果表明:病菌抗速克灵菌株频率平均为11.0％。抗性菌株中,85.7％属低抗水平,EC_(50)值在2.21～6.67μg·mL^(-1)之间。但菌株D_(1-3)抗药性水平极高,EC_(50)和EC_(95)分别达263.78和6000μg·mL^(-1)以上,EC_(95)值约是敏感菌株的1400倍。抗性菌株转管培养15和30代后,抗性水平仍较稳定。抗性菌株在菌丝生长速度、分生孢子与菌核形成以及对番茄致病力等方面无明显规律性,但高抗菌株D_(1-3)具有很强的生长、繁殖能力和致病力。

127 strains of Botrytis cinerea Pers. were isolated from 15 kinds of infected vegetable plants including cucumber , tomatot bean, eggplant, strawberry, hot pepper, lettuce, Chinese chive, towelgourd, garland chrysanthemum , romaine lettuce, celery, broad bean, seleng wormood and sweet corn in different areas of Jiangsu Province, China. The resistance of these isolates to procymidone was evaluated. On PSA media amended with 5 μg · mL-1 of pro-cymidone, the frequency of resistant isolates was on average 11. 0%. 85. 7% of them had low resistance, with 2. 21 -6. 67 ng · mL-1 of EC50. but the resistance of isolate D1-3 was much high, with 263. 78 μg · mL-1 of EC50 and more than 6 000 ng · mL-1 of EC85 respectively. Its EC95 value was about 1 400 times that of the sensitive isolate. Isolate D1-3 could maintain its resistance after 15 or 30 subcultures on the fungicide free PSA. In addition, there were no special differences in the mycelial growth, the formation of conidia and sclerotia, and the pathogenicity to tomato between resistant isolates and sensitive ones. But the resistant isolate D1-3 had significantly higher potential of growth, reproduction and pathogenicity.