β-伴大豆球蛋白水解产物中糖肽的分离和鉴定

Isolation and Identification of Glycopeptides in Soy Protein Hydrolysate

本研究对大豆蛋白水解产物中含糖的肽组分进行了分离和鉴定。从大豆蛋白中分离出相对纯度为75.5%、含糖量为3.23%的β-伴大豆球蛋白后,以β-伴大豆球蛋白为底物,用Alcalase进行酶解调制大豆肽。所得大豆肽经Sephadex G-25凝胶过滤,得到两个组分P1和P2,分子量较大P1含糖量为8.23%。用薄板层析对这一含糖的肽组分进行了鉴定,结果表明此含糖的肽为糖结合性肽。

The purpose of the study was to isolate the peptide containing carbohydrates from soy protein hydrolysate, andto identify the peptide -carbohydrate linkage. β- Conglycincin was prepared from soy protein. The relative purity and thecarbohydrate content of it was 75.5% and 3.23%, respectively. And thenβ- conglycincin was hydrolyzed with Alcalase. Elutionpattern of β- conglycincin hydrolysate was obtained after it was analyzed through gel chromatography (Sephadex G-25).According to the elution patterns the hydrolysate was divided into two fractions P1 and P2. Results showed that the fraction P1was peptides containing carbohydrate about 8.23%. Thin-chromatography indicated that the peptides (P1) were combined withthe carbohydrates. Namely, fraction P1 was glycopeptides.