酶联免疫检测甲型肝炎IgM抗体特异性诊断及临床特点

IgM Specific Diagnosis and Clinical Features of Enzyme-complex Immunologically Detected Hepatitis A Virus

检测甲型肝炎(简称甲肝)特异性1gM,是确定早期甲肝特异性诊断的可靠手段,我院探索酶联免疫吸附试验方法,经Abbott固相药盒对照,初步获得成功,共验测病毒性肝炎血清标本111份,65例阳性。

The detection of IgM, anti-HAV with enzyme linked immunosorbent assay technique (ELISA) was introduced in this article. 111 sera specimens of hospitalized patients with viral hepatitis were examined with this method. 65 of them were positive, in which 12 sera specimens were detected with both ELISA and RIA of Abbott simultaneously and with corresponding results. Another 51 cases were confirmed with immune adherence assay technique.116 serologieally confirmed hepatitis A cases were analysed. Of which 59 cases are males and 57 females. The average agewas 14.72+9.38 years old.TTT of 91 cases (78.4％) were abnormal, It seemed that, the earlier the abnormality appeared, the earlier the abnormality disappeared. After two weeks of illness the positives rate of anti-HAV IgM was 83.08%. After 1 to 2 months of illness the positives rate of anti-HAV IgG were 74.5%. The prognosis of this disease was good and no chronic case was found in our series.