虚寒状态大鼠肝脏能量代谢变化研究

Change of hepatic energy metabolism in rats with deficiency cold status

[目的]探讨氢化泼尼松诱导的虚寒大鼠肝脏能量代谢变化特点。[方法]48只SD雄性大鼠随机分为正常组和虚寒组,每组24只。虚寒组肌肉注射氢化泼尼松琥珀酸钠生理盐水溶液20 mg/kg,正常组注射生理盐水0.25 mL。注射第14、第21天晚上8点,正常组和虚寒组各12只,禁食不禁水12 h,次日上午8点,麻醉,腹主静脉取血,分离血清,全自动生化分析仪检测乳酸(LAC)。固定位置取一块儿肝脏,4%多聚甲醛固定,免疫组化法检测解偶联蛋白2(UCP-2)蛋白表达。其余肝脏,-80℃冻存,紫外分光光度法检测琥珀酸脱氢酶(SDH)和三磷酸腺苷酶(ATPase)。[结果]与同期正常组比较,虚寒组大鼠血清LAC含量、肝组织SDH活性在造模第14天和第21天均明显降低(P<0.05);肝组织中Na+-K+-ATP酶、Mg2+-ATP酶、Ca2+-ATP酶和Ca2+-Mg2+-ATP酶活性,肝脏UCP-2的阳性表达面积(Area)和积分光密度值(IOD)在造模第21天均明显降低(P<0.05,P<0.01)。[结论]氢化泼尼松诱导的虚寒状态动物模型肝脏能量代谢可能存在障碍。

Objective To investigate the change of hepatic energy metabolism in rats with deficiency cold induced by hydrocortisone. [Methods] Forty eight male SD rats were divided into two groups. The experimental group was intramuscularly injected with hydrocoriti-sone sodium succinate saline, 20 mg/kg and the control group was intramuscularly injected with saline, 0.25 mL. Five mL blood sample was collected from abdominal main vein on 15th day and 22th day. Serum LAC level were detected by automatic biochemical analyzer. The activities of Na+-K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, Ca2+-Mg2+-ATPase and SDH were detected by ultraviolet spectrophotometry. The protein expression of hepatic uncoupling protein 2 (UCP2) was detected by immunohistochemistry. [Results] Compared with those in control group, the activities of serum LAC and hepatic tissue SDH were significantly lower(P﹤0.05)on 14th day and 21th day, the activi-ties of hepatic tissue Na+-K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, Ca2+-Mg2+-ATPase, the positive expression areas and integral optical density of UCP-2 were significantly lower(P﹤0.05, P﹤0.01)on 21th day. The other indexes had no obvious change. [Conclusion] During the experimental process, the function of hepatic energy metabolism is significantly lower or disordered in animals with deficiency cold induced by hydrocortisone.