腰阳关穴位注射红茴香注射液对健康小鼠耐痛阈的影响

Effect of red fennel injection at Yaoyangguan acupoint on pain tolerance threshold in healthy mice

[目的]观察穴位注射红茴香注射液是否能提高健康小鼠的耐痛阈值，并比较穴位注射与肌肉注射的疗效差异，客观科学的评价穴位注射红茴香对痛阈的影响。[方法]将125只健康雌性小鼠随机分为穴位注射红茴香组（A组）、肌肉注射红茴香组（B组）、穴位注射生理盐水组（C组）、肌肉注射生理盐水组（D组）和空白对照组（E组），分别给予相对应的治疗措施，运用光热测痛仪，观察干预后1h至7d之内11个时间点小鼠的耐痛阈值。[结果]1）A、B组在所观察的时间点内耐痛阈的整体升高趋势较其他3组更为明显，并在注射后3~120 h内较C、D、E组间比较均有统计学差异（P﹤0.05）。A组于注射后24 h出现上升趋势，48 h达到顶峰，24~72 h之间维持较高水平，之后逐渐下降，144 h后接近正常水平。B组小鼠的耐痛阈在24~96 h之间明显升高，120 h后接近正常水平。2）C、D、E组所有时间点内组间比较均无统计学差异（P﹥0.05）。[结论]红茴香注射液有提高健康小鼠耐痛阈的作用，其效果在注射后24~96 h表现最为明显，生理状态下，穴位注射红茴香注射液尚未显示出其疗效优于肌肉注射。

Objective To observe the effect of red fennel injection for acupoint injection on pain tolerance threshold of healthy mice, compare the therapeutic differences of efficacy between acupoint and intramuscular injection and objectively and scientifically evaluate the efficacy of acupoint injection on pain tolerance threshold. [Methods] The 125 healthy female mice were randomly divided into red fennel acupoint injection group (A group), intramuscular red fennel group (B group), acupoint injection of saline group (C group), intra-muscular saline group (D group ) and blank control group (E group), receiving a corresponding treatment measure respectively. At 11 time points (1 h to 7 days) the analgesic effect was observed after the intervention with the light and heat pain measurement instrument. [Re-sults] 1)The overall trend of pain tolerance threshold of A and B group was more obvious at all the observation time points than the other three groups, and at the 3~120 h was significantly different when compared with C, D, E (P﹤0.05). At 24 h after injection group A had a upward trend, reaching the peak at the 48 h, maintaining a high level at 24~72 h continually. Then, it was decreased gradually, reaching the normal level at 144 h. In mice of group B the pain tolerance threshold was significantly higher at 24~96 h, closing the normal level at 120 h. 2)There were no statistically differences among C, D, E groups at all the observation points (P﹥0.05). [Conclusion] Red fennel in-jection can increase the pain tolerance thresholds of the healthy mice, with a obvious appearance at 24 to 96 hours. In the physiological condition, the acupoint injection of red fennel injection has not shown any superior effect than intramuscular injection.