升清降浊方对MSG肥胖大鼠脂代谢的影响及机制研究

Effects of Shengqing Jiangzhuo formula to improve the insulin sensitivity of MSG rats

[目的]研究升清降浊方(SQJZF)对L-谷氨酸钠(MSG)肥胖大鼠脂代谢的影响及其机制研究。[方法]复制MSG肥胖大鼠模型,随机分为模型组、阳性药罗格列酮组、阳性药非诺贝特组、SQJZF高剂量组(含生药6 g/kg)、SQJZF中剂量组(含生药3 g/kg)、SQJZF低剂量组(含生药1.5 g/kg);另取正常组大鼠8只。连续给药50 d后,分别检测大鼠肝脏脂质和血清游离脂肪酸的含量;实时定量聚合酶链反应(PCR)检测药物对MSG大鼠肝脏中过氧化物酶体增殖物激活受体α(PPARα)、脂蛋白脂肪酶(LPL)、载脂蛋白A-Ⅴ(Apo A-Ⅴ)、载脂蛋白C-Ⅲ(Apo C-Ⅲ)的基因表达水平。[结果]模型组MSG大鼠肝脏总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)和血清游离脂肪酸(NEFA)含量显著升高(P<0.05或P<0.01),肝脏高密度脂蛋白(HDL-C)含量显著降低(P<0.01);肝脏PPARα、LPL基因的相对表达量降低,Apo A-Ⅴ基因的相对表达量显著降低(P<0.01),Apo C-Ⅲ基因的相对表达量升高;给药50 d后,各给药组肝脏TC、TG、LDL-C和NEFA含量降低(P<0.05或P<0.01),肝脏HDL-C含量升高(P<0.05或P<0.01);对相关基因的相对表达均有一定的调节作用。[结论]SQJZF对MSG大鼠可能通过激活PPAR受体调控脂蛋白代谢来控制MSG肥胖大鼠脂代谢紊乱。

[Objective] To study the mechanisms and effects of Shengqing Jiangzhuo formula(SQJZF) to improve the lipid metabolism of MSG rats. [Methods] Copy the MSG obese rat models, which were randomly divided into model group, rosiglitazone group, fenofibrate group, three dose of SQJZF. Then choose 8 normal rats as the normal control group. Measure the levels of liqid in liver and the level of NEFA in serum after administration for 50 days. Real-time PCR detect the expression level of PPAR, LPL, Apo A-Ⅴ, A po C-Ⅲ in liver.[Results] Compared with the normal group, the model group improved the level of TC, TG, LDL-C in liver and the level of NEFA in serum and decreased the level of HDL-C in liver significantly(P<0.05 or P<0.01), and decreased the expression of PPAR, LPL, Apo A-Ⅴ significantly(P<0.01), and improved the expression of Apo C-Ⅲ. Compared with the model group, each of groups had some improvements of the level of liqid in liver and the level of NEFA and the level of genes expression. [Conclusion] SQJZF improve the lipid metabolism disorders in MSG rats which by activating the genes expression of PPAR.