葛根芩连汤对糖尿病大鼠早期视网膜病变影响的实验研究

Experimental study of Gegen Qinlian Tang on the impact of early retinopathy in diabetic rats

[目的]探讨葛根芩连汤对糖尿病大鼠早期视网膜病变的治疗作用。[方法]选取45只Wistar大鼠,链脲佐菌素(STZ)腹腔注射,制作糖尿病模型,糖尿病模型建立后随机分为DM组(糖尿病组)、DM+NS组(糖尿病生理盐水注射组)和实验组(DM+葛根芩连汤组)。模型建立后,DM组不做任何处理,实验组给予葛根芩连汤8.2 g/kg,DM+NS组注射等体积的生理盐水。对糖尿病大鼠的空腹血糖、血浆胰岛素、体质量、甘油三酯(TG)和高密度脂蛋白(HDL-C)的含量进行检测,以观察葛根芩连汤降糖降脂功效;通过免疫印迹(Westem blot)法检测视网膜蛋白激酶B(PKB)以及炎性相关因子——细胞间黏附分子-1(ICAM-1)的表达情况;利用苏木-伊红(HE)染色观察各组大鼠的超微结构;采用TUNEL法检测凋亡相关蛋白C-myc的凋亡指数。[结果]与DM组和DM+NS组比较,实验组大鼠空腹血糖明显降低、体质量明显增加,差异有统计学意义(P<0.05);与DM组和DM+NS组比较,实验组大鼠血胰岛素含量增加,组间比较差异有统计学意义(P<0.05);与DM组和DM+NS组比较,实验组大鼠TG明显降低而HDL-C明显升高(P<0.05)。通过Westem blot检测可见与DM组和DM+NS组比较,实验组大鼠PKB的表达明显增加、ICAM-1的表达明显下降,差异有统计学意义(P<0.05);光镜下HE染色观察到各组超微结构:DM组可见核固缩、浓染,核膜内陷,染色质边集,凋亡小体出现,实验组变化明显减轻(P<0.05);与DM组和DM+NS组比较,实验组中凋亡相关蛋白C-myc明显减少(P<0.05)。[结论]葛根芩连汤能够改善大鼠空腹血糖、血浆胰岛素及体质量等的变化,具有较好的降糖降脂作用,能通过增加PKB的表达及抑制糖尿病大鼠视网膜病变的炎症反应减轻或延缓糖尿病的发生和发展。

[Objective] To investigate the therapeutic effect of Gegen Qinlian Tang early stage of diabetic retinopathy in rats. [Methods]The 45 Wistar rats, streptozotocin(STZ) intraperitoneal injection, making diabetes were randomly divided into DM group, DM+NS group(saline injection group diabetes diabetic model) and the experimental group(DM +Gegen Qinlian group). After modeling, DM group received no treatment, and the experimental group was given Gegen Qinlian 8.2 g/kg, and DM +NS group was injected with an equal volume of saline. Diabetic rats, fasting blood glucose, plasma insulin, body weight, triglyceride(TG) and high density lipoprotein(HDL-C)content were detected to observe Gegen Qinlian hypoglycemic lipid-lowering efficacy; by Western blot method retinal protein kinase B(protein kinase B, PKB) and inflammation-related factors between a cell adhesion molecule 1(intercellular adhesion molecule1, ICAM-1)expression; HE staining used ultrastructure of rats in each group; with TUNEL assay of apoptosis-related protein C-myc in the apoptotic index. [Results] Compared with DM group and DM+NS, the experimental group was significantly lower fasting blood glucose, body weight increased significantly, statistically significant difference(P <0.05); the DM and DM +NS group, the experimental group of rats blood insulin content increased, the difference between groups was statistically significant(P<0.05); the DM and DM+NS group, the rats in the experimental group was significantly lower TG and HDL-C was significantly increased(P<0.05). By Western blot detection of visible and DM and DM+NS group, and PKB expression in rat experimental group increased significantly, and ICAM-1 expression was significantly decreased. The difference was statistically significant(P<0.05). HE staining was observed under light microscope ultrastructure groups:DM group pyknosis, stain, nuclear membrane retraction, chromatin margination, apoptotic bodies, changes in the experimental group was significantly reduced(P<0.05). The DM and DM+NS group, the experimental group of apoptosis-related protein C-myc decreased(P<0.05). [Conclusion] Gegen Qinlian Tang can improve fasting blood glucose, plasma insulin, and the change of body weight, with good blood glucose and lipids, by increasing the expression of PKB and suppress inflammation in diabetic rat retinopathy to reduce or delay the occurrence and development of diabetes.