HPLC法测定稳心颗粒中三七皂苷R1、人参皂苷Rg1、Rb1、Rd与党参炔苷的含量

Determination of Notoginsenoside R_1, Ginsenoside Rg_1, Rb_1, Rd and Lobetyolin in Wenxin Keli by HPLC

[目的]建立高效液相法（HPLC）同时测定稳心颗粒中三七皂苷R1、人参皂苷Rg1、Rb1、Rd与党参炔苷的含量。[方法]采用Agilent1260 DAD高效液相色谱仪,Amethyst C18色谱柱（4.6 mm×250 mm,4μm）,乙腈-水为流动相,检测波长为210 nm,流速1 m L/min。[结果]三七皂苷R1对照品在8247 mg/L线性关系良好,平均回收率为102.69%,RSD=2.74%（n=6）;人参皂苷Rg1对照品在14422 mg/L线性关系良好,平均回收率为98.72%,RSD=1.85%（n=6）;党参炔苷对照品在1.542 mg/L线性关系良好,平均回收率为97.69%,RSD=1.94%（n=6）;人参皂苷Rb1对照品在2.65847 mg/L线性关系良好,回收率为102.11%,RSD=1.96%（n=6）;人参皂苷Rd对照品在8255 mg/L线性关系良好,平均回收率为99.66%,RSD=2.49%（n=6）。[结论]本实验中三七皂苷R1、人参皂苷Rg1、Rb1、Rd和党参炔苷的含量测定方法稳定可靠,可作为稳心颗粒中三七皂苷R1、人参皂苷Rg1、Rb1、Rd与党参炔苷含量测定方法。

[Objective] To establish a HPLC method for simulataneous determination of Notoginsenoside R1（1）, Ginsenoside Rg1（2）,lobetyolin（3）, Ginsenoside Rb1（4）, Ginsenoside Rd（5） in Wenxin Keli by HPLC. [Methods] The HPLC was conducted on Amethyst C18column（4.6 mm×250 mm, 4 μm） with acetonitrile-water solution as themobile phase. The detection wavelength was 210 nm, and flow rate was 1 m L/min. [Results] Linearity of each standard was established within the concentration range of 8247 mg/L for 1, 14422 mg/L for 2,1.542 mg/L for 3, 26.5847 mg/L for 4 and 8255 mg/L for 5. The average recovery（n=6） of the compound 1-5 was 102.69% with RSD of 2.74%, 98.72% with RSD of 1.85%, 97.69% with RSD of 1.94%, 102.11% with RSD of 1.96%, 99.66% with RSD of 2.49%respectively. [Conclusion] The treatment of samples was stable and reliable. The method can be used for determination of notoginsenoside R1, lobetyolin, ginsenoside Rg1, Rb1 and Rd in Wenxin Keli.