摩腹手法对睡眠剥夺大鼠脑内神经递质及Orexin-A的影响

Effect of abdominal massage therapy on cerebral neurotransmitter and Orexin-A in sleep deprivation rats

[目的]观察和探讨摩腹手法对睡眠剥夺大鼠脑内神经递质及Orexin-A水平的影响。[方法]将Wistar大鼠30只随机分为正常对照组、模型对照组和摩腹手法组各10只,采用改良多平台水环境法对后两组大鼠进行睡眠剥夺造模,造模后正常对照组和模型对照组大鼠不作干预,摩腹手法组大鼠给予每日1次的摩腹治疗,持续14 d后,检测比较各组大鼠脑内5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)、γ-氨基丁酸(GABA)和食欲素A(Orexin-A)的水平。[结果]与正常对照组相比,模型对照组大鼠脑内5-HT、DA、NE、GABA和Orexin-A的水平均发生显著变化(P<0.05)。与模型对照组相比,摩腹手法组大鼠脑内5-HT水平显著回升,DA、NE和Orexin-A水平均明显回落(P<0.05),而GABA水平无统计学差异(P>0.05)。[结论]摩腹手法可通过调节大鼠脑内5-HT、DA、NE和Orexin-A的水平,起到调节动物睡眠和觉醒状态的作用。

[Objective] To observe and explore the effect of abdominal massage therapy on cerebral neurotransmitter and Orexin-A in sleep deprivation rats. [Methods] The 30 Wistar rats with 10 in each group were randomly divided into normal control group,model control group,and abdominal massage therapy group. Adopting a modified multi-platform water environment method for sleep deprivation in the latter two groups of rats,Rats in the normal control group and model control group did not interfere after modeling. The rats in the abdominal massage therapy group were given a daily abdominal massage therapy. After 14 days,compare the levels of 5-HT,DA,NE,GABA,and Orexin-A in the brains of rats in each group. [Results] Compared with normal control group,the levels of 5-HT,DA,NE,GABA,and Orexin-A in the brain of the model control group were significantly changed(P <0.05). Compared with the model control group,the level of 5-HT in the brain of the rats in the abdominal massage therapy group was significantly increased,and the levels of DA,NE,and Orexin-A significantly decreased(P<0.05),while there was no significant difference in GABA levels(P>0.05). [Conclusion]Abdominal massage therapy can regulate the levels of 5-HT,DA,NE,and Orexin-A in the brain of rats and play a role in regulating sleep and wakefulness in animals.