国产毛细管电泳仪检测血清肌酐

Determination of creatinine in human sera with micellar electrokinetic capillary electrophoresis by the homemade instrument

目的 :用国产毛细管电泳仪建立毛细管电泳测定血清肌酐的方法。方法:采用毛细管胶束电动色谱法 ,用国产TH -2000毛细管电泳仪进行血清肌酐的测定。电泳缓冲液为硼酸盐缓冲液 (20mmol/L,pH9.0) ,含120mmol/L十二烷基磺酸钠 (SDS)。紫外检测波长235nm。结果:肌酐在25 0～1600μmol/L范围内有良好的线性 ,最小检出限为12 3μmol/L(信噪比=3) ;本法CV 批内 、CV 日内 均小于5 % ;低、中、高浓度的回收率分别为96 0 %、99 4 %、96 5 % ;与全自动生化分析仪 (碱性苦味酸法 )测定结果对比 ,相关系数r=0.9977 ,回归方程Y=0.971X -16.48;尿素、尿酸、咖啡因、维生素C、血红蛋白、胆红素、脂血等对本方法无干扰。结论:该法简单 ,分离效果好 ,上样量少 ,成本低 ,分析速度快 。

Objective:To develop a new method for determining creatinine in humanserum using capillary electrophoresis(CE)by the homemade instrument.Methods:Creatinine in human serum was determined with micellar electrokinetic CE and detected at235nm.The running buffer for analyses was120mmol/L SDS_20mmol/L borate,pH9.0.Results:The linear conˉcentration of creatinine was25.0～1600μmol/L with detection limit of12.3μmol/L.The CV values for within_run_assay and within_day_assay were all less than5%.The recoveries of creaˉtinine at different levels were96.0%,99.4%and96.5%,respectively.Comparing this method(Y)with Jaffe method(X),it obtained the regression equation.Y=0.971X-16.48,r=0.9977.Urea,uric acid,caffeine,vitamin C,hemoglobin,bilirubin and lipoprotein had no interfernce with this procedure.Conclusion:CE method is simple,rapid,separation efficient and suitable for the analysis of creatinine in serum clinically.