内皮素受体基因在心力衰竭患者外周血的表达及意义

Expression of endothelin receptors gene in patients with congestive heart failure

目的 :研究充血性心力衰竭 (心衰 )患者血浆内皮素 (ET)水平和ET受体基因在其外周血单个核细胞表达情况。方法 :在 36例心衰 (心衰组 )患者和 12例健康人 (对照组 )中分别采用放免法测定血浆ET水平、逆转录聚合酶链反应检测ET受体基因在其外周血单个核细胞的表达。结果 :心衰组血浆ET水平〔(4 .90± 1.96 )pmol L〕明显高于对照组〔(1.76± 0 .82 )pmol L〕(P <0 .0 1)。ET受体基因表达阳性率在两组差异无显著性意义(P >0 .0 5 ) ,半定量后 ,心衰组ETA受体 (0 .82± 0 .2 6 )表达水平明显高于对照组 (0 .5 9± 0 .17) (P <0 .0 1)。结论 :ET及其A受体与心衰的发生。

Objective:To investigate the levels of plasma endothelin and the expression of endothelin receptors gene in patients with congestive heart failure.Methods:The radio immunologic method was used to measure the plasma concentration of ET in 36 CHF pationts and 12 healthy (control). RT PCR was used to check the expression of CT recoptor gene in peripheral blood mononuclear cell. Results:Plasma endothelin of patients ( 4.90 ± 1.96 ) pmol/L was higher than that in healthy control ( 1.76 ± 0.82 ) pmol/L (P< 0.01 ). There was no significant difference in the positive rate expression of endothelin receptor gene between patients and health control (P> 0.05 ). There was no significant difference for ET receptor gene expression beween 2 groups ( 0.59 ± 0.17 ) (P< 0.01 ).Conclusion:Plasma endothelin levels was elevated and endothelin A receptors were upregulated in patients with congestive heart failure. The ET system plays a key role in the pathogenesis of congestive heart failure.