四倍体刺槐优良无性系间组织培养比较

Studies on Buds Proliferation between Different Types of Tetraploid Locust Trees in Vitro for Rooting

通过对四倍体刺槐K_2、K_5 无性系芽增殖基本培养基和激素配比比较试验研究,其结果表明:K_2、K_5无性系芽增殖各指标在不同培养基、不同激素浓度配比情况下生长趋势表现均基本一致;K_2、K_5无性系适宜的基本培养基为MS;芽增殖最佳激素配比为:6-BA1.0 mg/L+NAA0.5 mg/L+IBA 0.5 mg/L。

Studies on buds proliferation between different types of tetraploids locust trees in vitro were performed to select the optimal medium and the supplements of different growth regulators and their combinations f6r proliferation. The results showed; both K2 and K5 strain present the same regularity in average stat. of buds proliferation; MS medium was the optimal medium suitable to buds proliferation of the tree in vitro. Different plant growth regulators and their combinations were supplemented to MS basic medium to check their influence on shoots culture. By multi-variance analysis. It could inferred that the optimal combination was 6-BA1. 0 mg/L + NAA0. 5 mg/L + IBA 0.5 mg/L.