穴贴定喘膏对过敏性哮喘豚鼠IL_3、IL_5和IgE的影响

Effect of Xuetie Dingchuang Plaster on the Levels of IL_3, IL_5 and IgE in Guina Pigs with Allergic Asthma

目的 探讨穴贴定喘膏治疗过敏性哮喘模型豚鼠的作用机理。方法 用 10 %卵蛋白液给豚鼠致敏 ,2周后再用1%的该溶液雾化吸入 ,以诱发豚鼠哮喘 ,然后将发作的哮喘豚鼠随机分为模型组、先声咳喘宁组 (7 5ml·kg-1)、穴贴定喘膏组(1g·kg-1、3g·kg-1) ,同期另设一正常组。先声咳喘宁组豚鼠灌胃给药 ,穴贴定喘膏组豚鼠经皮给药。每日给药 1次 ,连续 7日。其间在给药 1h后 ,诱发哮喘 1次。 1周后取豚鼠血清 ,测定IL3 、IL5和IgE水平。结果 穴贴定喘膏能降低血清IL3 、IL5和IgE ,与模型组比较具有显著性差异 (P <0 0 5 )。结论 穴贴定喘膏治疗过敏性哮喘作用机理与降低IL3 、IL5和IgE有关。

Objective\ To investigate the antiasthmatic mechanisms of Xuetie Dingchuang Plaster in guina pigs with allergic asthma.Method\ The guina pigs were sensitized with a 10% albumin solution, and 2 weeks later a further 1% of this solution was atomized and inhaled to induce asthma. Then the qualified guina pigs were randomly divided into 3 gropus:model, kechuanning(7 5 ml·kg -1 , ig ), Xuetie and Dingchuang Plaster(1 g·kg -1 ,3 g·kg -1 , transdermal). Besides a normal group was added at the same time. All interventions were adminestered once a day for 7 days and 1 h after the adminestration asthma was induced by albumin solution. The serum levels of IL 3, IL 5 and IgE were measured at the end of the experiment. Result\ Xuetie Dingchuang Plaster significantly decreased the levels of serum IL 3, IL 5, and IgE compared with the model group( P <0 05). Conclusion\ The role of Xuetie Dingchuang plaster on antiasthma is possibly related to its effect of decreasing serum levels of IL 3, IL 5 and IgE.