摘要
以非洲菊南燕F1种子无菌萌发的种子苗作外植体,在含不同植物激素的培养基上进行丛生芽的诱导、芽的继代增殖、芽生根与移栽,以快速获得再生植株.结果表明:丛生芽诱导培养基以MS+BA1.5mg·L-1+IBA0.2mg·L-1较好,诱导系数达到7;增殖培养基以MS+BA0.5mg·L-1+NAA0.1mg·L-1较好,增殖系数高达10,且增殖芽生长好;生根培养基以1/2MS+IBA0.2mg·L-1最佳,生根率达100%,平均生根数量多,幼苗生长势旺.
This research, selecting seedlings of Gerbera jamesonii hybrids 'Nan Yan' bourgeoned in germfree conditions as explants, makes a study of buds inducement and reproduction, root differentiation and young plant rootage in different culture media. The results show that MS+BA 1.5 mg.L-1+IBA 0.2 mg.L-1 is good for buds inducement and the coefficient of inducement is 7, that MS+BA 0.5 mg.L-1+NAA 0.1 mg.L-1 is suitable for buds reproduction, the coefficient of reproduction is up to 10 and buds grow very well, and that 1/2 MS+IBA 0.2 mg.L-1 is best for rootage, the rooting rate is 100%, the average number of rooting is high and the young plants grow very well.
出处
《中南林学院学报》
CSCD
2003年第5期45-48,共4页
Journal of Central South Forestry University
基金
中南林学院青年科学基金资助项目"非洲菊的组织培养与快速繁殖"的内容.
关键词
园艺学
花卉
非洲菊
组织培养
无菌苗
horticulture
flowers and plants
Gerbera jamesonii hybrids
tissue culture
germfree seedling
