肺挫伤致肺水肿患者血浆中E-SLT,IL-8,TNF-α和ET-1的变化(英文)

STUDY ON E - SLT、IL - 8 、TNF - α AND ET - 1 IN PLASMA OF 24 PATIENTS WITH LUNG CONTUSION

目的:本研究观察胸部外伤所致肺水肿病人血中E-选择素、TNF、白细胞介素-8和内皮素的变化。方法:用固相双夹心酶联免疫吸附法和放免技术共测定了24例胸部外伤病人在治疗前后血中E-选择素、肿瘤坏死因子、白细胞介素-8和内皮素浓度。结果:临床肺水肿病人血中E-选择素、肿瘤坏死因子、白细胞介素-8和内皮素的浓度在发病时明显升高,与正常对照组比较,差异有显著性(P<0.001)。治愈后恢复正常,治疗后各项指标与正常对照组比较差异元显著性(P>0.05),但治疗前后差异有显著性(P<0.001)。结论:E-选择素、肿瘤坏死因子、白细胞介素-8和内皮素在肺水肿发病机制中起着重要,随着这些因子的升高出现肺水肿并逐渐加重,这些因子降低后肺水肿逐步消失。因此对这些因子的检测有助于估计病情和评价疗效。

Objective:To study the effect of ET- 1 ,TNF - α, E - SLT and IL - 8 in plasma of 24 patients with lung contusion. Methods :Double antibody sandwich quantitative ELISA method was used to measure the concentration of E - SLT, and radioimmunoassay (RI A) were used to measure the concentration of TNF - α , ET - 1, and IL -8 in 24 patients with lung contusion and 10 normal control subjects. Results:Compared with those in normal compared group, the plasma concentration of E - SLT, ET- 1, TNF- α and IL - 8 in lung contusion increased significantly, there were also significant difference of the concentration of ET - 1, TNF - α, E - SLT and IL - 8 in 24 patients between pretreatment and post - treatment. Conclusions: ET - 1, TNF - α, E - SLT and IL - 8 might play an important role in lung contusion caused by lung edema. Assay of ET - 1, TNF - α, E - SLT and IL - 8 is very useful to observe advance of lung contusion and judge therapy effect.