乌司他丁预防小鼠术后腹腔粘连实验研究(英文)

PREVENTION OF POSTOPERATIVE PERITONEAL ADHESIONS BY ULINASTATIN IN MICE

目的:研究乌司他丁预防小鼠术后腹腔粘连的能力。方法:以BALB/c小鼠为动物模型,经过一同一标准的腹膜损伤手术后,60只BALB/c小鼠随机分成两组:乌司他丁治疗组,生理盐水对照组,分别在关腹前腹腔内灌注乌司他丁及生理盐水。术后20d将小鼠处死,观察腹腔内粘连的情况,通过对粘连的程度、位置以及粘连类型的综合评价得出粘连分数。结果:乌司他丁治疗组的平均粘连分数(1.28±0.31)明显低于对照组(2.33±0.47),2-3级粘连发生率(23.3％)比对照组(76.7％)亦有明显减低(P<0.01)。无1例小鼠出现腹壁切口疝。结论:乌司他丁能够预防术后腹腔粘连形成,且对伤口愈合无明显影响。

Objective:To assess the ability of ulinastatin to prevent postoperative peritoneal adhesions formation in mice.Methods:60 BALB/c mice were randomly divided into two groups: ulinastatin group (n = 30) and control group (n= 30). After an identical peritoneal injury operation, mice were treated intraperitioneally with ulinastatin or saline at the time of abdominal closure. The mice were killed 20 days after operation, then the abdominal incisions and the development of intraabdominal adhesions were observed. Adhesion scoring was based on an overall assessment of the extent, location and adhesions type. Results: Ulinastatin group had significantly lower adhesion scores (1.28±0.31) than the control group (2.33±0.47). When two groups were analyzed for the presence of grade 2 -3 adhesions (advanced significant adhesions), the ulinastatin group had significantly lower incidence (23.3%) than control group (76. 7%). No incisional hernia was found in abdominal wall in all cases. Conclusions: The in-traperitoneal administration of ulinastatin could inhibit postoperative adhesion formation, and has no obviously side-effect on incision healing.