摘要
[目的 ]运用基因芯片技术 ,比较正常大鼠和糖尿病大鼠肝脏中细胞色素p45 0 2E1(CYP2E1)基因表达的差异。[方法 ]对雄大鼠腹腔注射链脲佐菌素 (STZ) ,并予以高脂饮食制造 2型糖尿病模型 ;从其肝脏抽提总RNA ,进行反转录 ,并用Cy3 dUTP和Cy5 dUTP分别对正常组与实验组探针进行荧光标记 ,探针与基因表达谱芯片杂交 ,结果由扫描仪扫描并用软件进行分析统计。 [结果 ]糖尿病组与对照组的肝脏相比 ,Cy5 /Cy3比值为 10 0 82 ,有统计学意义 ,CYP2E1mRNA表达升高。[结论 ]
To compare the expression of CYP2E1 in liver of Type 2 diabetic rats with matched non diabetic controls by cDNA microarray \ Type 2 diabetes for the rats was induced by streptozotocin (STZ), and then feeding with high fat dietary The probes were prepared by labling normal tissue mRNA and tissue mRNA of diabetic rats with Cy3 dUTP and Cy5 dUTP separately The array was then hybridized against the cDNA probe mixture and the fluorescent signals were scanned \ The ratio of Cy5/Cy3 is 10 082 The levels of CYP2E1 mRNA were significantly elevated in liver of diabetic rats \ Diabetes increases the expression of CYP2E1mRNA
出处
《海峡预防医学杂志》
CAS
2003年第5期8-10,共3页
Strait Journal of Preventive Medicine
