二硫代氨基吡咯烷和维生素C对急性胰腺炎模型大鼠胰肝细胞粘附分子及超氧化物歧化酶表达的影响

Effects of pyrrolidine dithiocaramate and Vit C on ICAM -1 and SOD gene expression in rats with acute pancreatitis

目的 :探讨二硫代氨基吡咯烷 (PDTC)和维生素C(VitC)对急性胰腺炎 (AP)模型大鼠胰腺及肝脏中超氧化物歧化酶 (SOD :Mn -SOD ,Cu ,Zn -SOD)和细胞粘附分子 (ICAM - 1)表达的影响。方法 :经十二指肠壁进入胰管开口处 ,逆行加压注入 3%牛磺脱氧胆酸钠复制大鼠AP模型。模型随机分为 3组 :AP +生理盐水 (NS)组、AP +PDTC组和AP +VitC组 ,并在复制模型手术后 10min和 5h按分组给药。另设假手术组。各实验组于术后 1、5、10h取胰腺和肝脏 ,采用RT -PCR方法测定其ICAM - 1及Mn -SOD、Cu ,Zn -SOD的表达。结果 :AP +NS组胰腺和肝脏ICAM - 1表达高于假手术组 (P <0 0 5 ) ,胰腺Cu ,Zn -SOD表达低于假手术组 (P <0 0 5 )。AP +PDTC组和AP +VitC组胰腺Mn -SOD、Cu ,Zn -SOD表达高于AP +NS组 (P <0 0 5 ,P <0 0 1)。AP +PDTC组胰肝ICAM - 1表达低于AP+NS组 (P <0 0 5 )。结论 :结果提示 ,PDTC和VitC缓解AP的作用机制在于不仅它们自身是抗氧化剂 ,而且它们促进SOD的表达 ,同时PDTC还具有抑制ICAM - 1表达的作用。

AIM: To investigate the effects of pyrrolidi ne dithiocaramate (PDTC) and vitamin C (Vit C) on the intercellular adhesion mol ecule 1 (ICAM-1) gene e xpression and the superoxide dismutase (Mn-SOD,Cu,Zn-SOD) gene expression in a r at model of acute pancreatitis (AP). METHODS: Rat AP model was induced by retr ograde injection of 3% sodium taurocholate in the bilio-pancreatic duct. The AP rats were divided into three groups: the normal saline (NS) group, the PDTC grou p and the Vit C group. The three groups were separately administered NS, PDTC a n d Vit C at 10 min and 5 h after operation. Rat sham operation (SO) group was su bj ected to laparotomy only with no further treatment. The levels of ICAM expressio n, Mn-SOD and Cu, Zn-SOD expression in pancreas and liver were determined by R T-PCR at 1, 5 and 10 h after the induction of AP. RESULTS: Compari ng with SO gr oup, the NS group showed a higher expression of ICAM-1 and a lower expression of Mn-SOD both in pancreas and in liver, and a lower expression of Cu, Zn-SOD in p a ncreas. PDTC treatment reduced the ICAM-1 expression and increased the Mn-SOD an d Cu, Zn-SOD expression to a certain degree comparing with NS group, especially at 5 h. Vit C treatment also increased the Mn-SOD and Cu, Zn-SOD expression . But no obvious suppressive effect on ICAM-1 expression was observed in Vit C group. CONCLUSIONS: PDTC and Vit C have the effects of enhancing Mn-SOD and Cu, Zn-SOD ex p ression. PDTC has an effect of inhibiting ICAM-1 expression in the pancreas and liver of rats with AP, whereas Vit C dose not. The results suggest that one of the mechanisms of PDTC and Vit C treating AP not only anti-oxidate directly by t hemselves, but also enhance Mn-SOD and Cu, Zn-SOD expression, and that the anti- inflammatory effect of PDTC is involved in the suppression of ICAM-1 expression.