红景天对烫伤后多器官功能不全综合征作用的一氧化氮机制

The possible nitric oxide mechanism of Rhodiola on the multiple organ dysfunction syndrome after burns

目的 :观察红景天、一氧化氮 (NO)、血红蛋白 (HB)对兔烫伤早期多器官功能不全综合征 (Multipleor gandysfunctionsyndrome,MODS)的作用 ,并分析其作用机理。方法 :动物分假烫组 (SB) ,烫伤组 (B) ,口服红景天对照组 (R) ,烫伤红景天治疗组 (BR)。检测各组血流动力学指标 ;测定肺泡灌洗液蛋白及血浆总蛋白 ,计算肺通透指数 ;测定反映心、肺、肝、肾、血液功能的一系列指标。用Griess法测定血清、肺泡灌洗液中NO含量。测定血清中HB含量。结果 :①B组烫伤后 4 8h(B48h)肌酸磷酸激酶、谷丙转氨酶、尿素氮均明显上升 ,达到MODS标准 ,肺功能下降 ,心脏指数减低 ,动物发生MODS。B48h动物血清及支气管肺泡灌洗液NO含量均明显多于SB组。②BR组烫伤 4 8h(BR48h)后动物心脏指数、心肌酶、尿素氮明显高于或低于B48h组 ,血清及支气管肺泡灌洗液NO含量均明显多于B48h组。③B组烫伤后即刻 (B0h)血清中HB含量明显高于SB组及B48h组、BR48h组 ,NO含量反之 ;BR组烫伤后即刻(BR0h)血清中HB含量明显高于R组及BR48h组 ,NO含量反之。结论 :①动物烫伤即刻血清中NO含量显著减少 ,其清除剂HB含量显著增高。②红景天促使烫伤动物NO含量增高 ,增加器官血灌流量 ,可能是其防止MODS发生的机制之一。

AIM: To study the effects of Rhodiola(Rho), nitric oxid e (NO), hem oglobin (HB) on the multiple organ dysfunction syndrome(MODS) in early stage aft er burn in rabbits. METHODS:The rabbits were divided into the sh am burn group (SB), burn group (B), orally taken Rho group (R), burn and Rho therapy group (BR ). The changes of hemodynamics were monitored. The index of pulmonary permeabili ty was calculated. These data reflected separately the functions of heart, live r, lung, kidney and blood coagulation system were also determined. NO contents i n ser um and bronchoalveolar lavage fluid (BALF) were measured by Griess method. The l e vels of serum HB were measured. RESULTS:① The dysfunctions of hear t, liver and kidney achieved the criterion of MODS in group B at 48 h postburns (B 48 h ). The N O content of group B significantly increased in serum and BALF at B 48 h . ② The cardiac index (CI) and creatine phosphokinase (CK), urea nitrogen (BUN) markedly raised or decreased in group BR at 48 h postburns (BR 48 h ) than B 48 h . The NO cont ents in serum and BALF markedly raised. ③ HB contents in serum markedly raised in group B and BR at 0 h postburns (B 0 h , BR 0 h ) than group SB, B 48 h , R, BR 48 h , but NO was reverse. CONCLUTIONS: ① HB contents in serum markedly raise d at 0 h post burns, but NO was reverse. ② Rho promoted the increases of NO synthesis and the blood perfusion of organs, which might be one of mechanisms to prevent the devel opment of MODS.