茶多酚对高脂环境中THP-1细胞核因子-κB、转化生长因子-β_1 mRNA表达的影响

Effect of tea-polyphenols on the activation of NF-κB an d the expression of TGF-β_1 mRNA in THP-1 cells incubated with VLDL, LDL or ox-LDL

目的 :观察茶多酚 (TP)对单核细胞源性泡沫细胞核因子 -κB(NF -κB)、转化生长因子 - β1(TGF -β1)mRNA表达的影响。方法 :分别用极低密度脂蛋白 (VLDL)、低密度脂蛋白 (LDL)、氧化低密度脂蛋白 (ox -LDL)诱导人髓系白血病单核细胞株THP - 1细胞向泡沫细胞转化 ,加入TP干预后检测细胞NF -κB的核移位率、TGF - β1mRNA阳性指数、细胞内胆固醇含量等。结果 :茶多酚浓度为 0 4 - 4 0 μg/L时细胞NF -κB的核移位率、TGF - β1mR NA阳性指数、细胞内胆固醇含量等均低于未用TP干预的细胞 (P <0 0 5 )。结论 :一定浓度的TP对高脂环境中单核-巨噬细胞NF -κB活化、TGF - β1mRNA表达有抑制作用 ,并阻止泡沫细胞形成。

AIM: To investigate the effect of tea-polyphe nols (TP) on the activation of NF-κB and the expression of TGF-β 1 mRNA in TH P -1 cells (a human acute monocytic leukemia cell line). METHODS: TH P-1 cells were incubated with the different concentrations of TP, VLDL, LDL or o x-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-κB was detected with immunohistochemistry technique, the positive index of the TGF-β 1 mRNA ex p ression was detected by hybridization in situ, and accumulation of total cholest erol (TC) in cells incubated with 0 4-40 μg/L TP was determined with oxidase a ssay. RESULTS: The nuclear malposition rate of NF-κB, the posit ive index of the TGF-β 1 mRNA expression and TC in THP-1 cells incubated with 0 4-40 μg/L of TP were lower than those with 0 μg/L of TP in TP-V group, TP-L group and TP-O ( P <0.05). The differences of these markers in THP-1 cells i ncubated with more than 40 μg/L TP in TP-V group, TP-L group and TP-O were n ot statistically significant, compared with TP-C group ( P >0.05). CONCLUSION: TP inhibited the activation of NF-κB, the expressio n of TGF-β 1 mRNA and the foam cell formation in the mono-macrophage.