附子不同组分抗急性心力衰竭大鼠的实验研究

Study on the effects of different components of Radix Aconiti Lateralis on rats with acute heart failure

从血流动力学和神经体液因子两方面探讨附子不同组分对大鼠急性心力衰竭模型的治疗作用。采用盐酸普罗帕酮复制急性心力衰竭模型,记录造模前后以及给药后5、10、20、30、60 min大鼠心脏频率(HR)、左心室最大上升速率(+dp/dt_(max))和左心室最大下降速率(-dp/dt_(max))值的变化,并测定血清中Ang-Ⅰ、Ang-Ⅱ、TNF-α和ANP含量。结果显示,正常大鼠造模后,其HR和+dp/dt_(max)明显降低,-dp/dt_(max)明显升高,且Ang-Ⅰ、Ang-Ⅱ、TNF-α、ANP均有升高趋势。与模型组比较,总生物碱组和水溶性生物碱组可不同程度的升高模型大鼠各时间点的HR、+dp/dt_(max),降低-dp/dt_(max);且总生物碱组、水溶性生物碱组、脂溶性生物碱组和多糖组均有一定降低模型大鼠血清中Ang-Ⅰ、Ang-Ⅱ、TNF-α、ANP水平含量的趋势。结果表明总生物碱对改善血流动力学作用最强,其次是水溶性生物碱,且两者均能显著降低血清中与心力衰竭有关的神经体液因子,有效治疗急性心力衰竭,由此可见,附子水溶性生物碱以及总生物碱是其发挥强心作用的主要物质基础。

To study the effects of different components of Radix Aconiti Lateralis on rats with acute heart failure(AHF)in hemodynamics and neurohumoral factors.Use propafenone hydrochloride to copy the model rats of AHF.Then,observe and record the change of HR,+dp/dtmax and-dp/dtmaxafter administration 5,10,20,30,60 min,and measure the concentration of Ang-Ⅰ,Ang-Ⅱ,TNF-α,ANP by blood sampling after 60 min by ELISA.After modeling with propafenone hydrochloride,HR,+dp/dtmax were significantly decreased and-dp/dtmax was significantly increased,and Ang-Ⅰ,Ang-Ⅱ,TNF-α,ANP were trend to rise.Compared with the model group,total alkaloids and water-soluble alkaloids can increase the HR,+dp/dtmaxand decrease the-dp/dtmax in varying degrees at all points of time;total alkaloids,water-soluble alkaloids,fat-soluble alkaloids and polysaccharides had the tendency to reduce the concentration of Ang-Ⅰ,Ang-Ⅱ,TNF-α,ANP,which were increased by modeling.In conclusion,the total alkaloids had the most significant effect of improving hemodynamics,followed by the water-soluble alkaloids,both of which could affect the neurohumoral factors associated with heart failure.Thus,it can be seen that the water-soluble alkaloids and total alkaloids are the main material basis in playing the role of cardiac.