小檗碱对乳腺癌MDA-MB-231细胞增殖抑制作用及其分子机制研究

Effect of berberine on the proliferation of breast cancer MDA-MB-231 cells and its molecular mechanism

本研究的目的是为了探索小檗碱对乳腺癌MDA-MB-231细胞增殖的影响以及阐明小檗碱促乳腺癌细胞凋亡的分子机制。在实验过程中,我们通过MTT检测小檗碱对乳腺癌MDA-MB-231细胞增殖的抑制作用,采用Annexin-V/PI染色定量考察小檗碱对肿瘤细胞凋亡的影响,运用Western Blot实验检测肿瘤相关通路蛋白表达来进行研究。实验表明小檗碱对乳腺癌MDA-MB-231细胞增殖具有抑制作用,使细胞中自噬因子Beclin 1表达增加,诱导细胞自噬泡的形成,导致肿瘤细胞发生凋亡。综上说明小檗碱是通过抑制AKT-mTOR通路,诱导MDA-MB-231细胞的自噬以及凋亡,从而发挥抗肿瘤作用。

This study aims to explore the effect of berberine on the proliferation of breast cancer MDA-MB-231 cells and to explain the molecular mechanism of berberine promoting apoptosis in breast cancer cells.During the experiment,MTT was used to detect the inhibitory effect of berberine on the proliferation of breast cancer MDA-MB-231 cells.Then,Annexin-V/PI staining was used to quantitatively investigate the effect of berberine on tumor cell apoptosis.Finally,Western Blot assay was used to detect tumor-associated pathway protein expression for study.The results showed that berberine inhibited the proliferation of breast cancer MDA-MB-231 cells,which increased the expression of autophagy factor Beclin 1 in the cells.Further,the formation of autophagic vacuoles was induced,resulting in apoptosis of tumor cells.In summary,berberine exerted anti-tumor effects by inhibiting autophagy and apoptosis of MDA-MB-231 cells by inhibiting the AKT-mTOR pathway.