黄芩苷对酯多糖损伤AC16心肌细胞的保护作用及机制研究

Protective effect and mechanism of Baicalin on lipopolysaccharide-induced AC16 cardiomyocytes

本研究主要探讨黄芩苷对酯多糖损伤AC16心肌细胞的保护作用。设置对照组、LPS组、黄芩苷高、中、低剂量组、黄芪总黄酮组,共同培养6 h,检测细胞生存率及生存状况;荧光素法检测细胞内ATP浓度变化; Western blot检测各组细胞中Cyt C、Apaf-1、caspase-3、caspase-9表达。结果发现与LPS组相比,黄芩苷高、中、低剂量组及黄芪总黄酮组细胞生存状况及生存率明显改善,细胞内ATP浓度升高,凋亡率降低,细胞内Cyt C、Apaf-1、caspase-3及caspase-9表达均降低。说明黄芩苷对酯多糖损伤的AC16心肌细胞具有保护作用,其机制可能与降低Cyt C/Apaf-1/caspase-3/caspase-9通路表达有关。

This study mainly discussed the protective effect of baicalin on AC16 cardiomyocytes injured by Lipopolysaccharide.The experiment was divided into control group,LPS group,baicalin high,medium and low dose groups,total flavonoids of astragalus membranaceus group and cultured for 6 hours to test cell survival rate and survival status;The firefly luciferin method detects changes in intracellular ATP concentration;The expressions of Cyt C,Apaf-1,caspase-3 and caspase-9 in each group were detected by Western blot.The results showed that compared with the LPS group,the cell survival rate and survival status of the high,middle and low doses group of baicalin and the total flavonoids of astragalus membranaceus group were significantly improved,the intracellular ATP concentration increased,and the apoptotic rate decreased.The expression of Cyt C,Apaf-1,caspase-3 and caspase-9 was decreased in cells.It indicated that baicalin has protective effect on AC16 cardiomyocytes damaged by LPS,and its mechanism may be related to the decrease of Cyt C/Apaf-1/caspase-3/caspase-9 pathway expression.