HPLC-UV-ELSD法同时测定黄芪中黄芪皂苷和黄酮类成分

Simultaneous determination of astragaloside and flavonoids components in astragali radix using HPLC-UV-ELSD

建立HPLC-UV-ELSD法同时测定黄芪中黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪皂苷Ⅲ、黄芪甲苷、毛蕊异黄酮葡萄糖苷、刺芒柄花苷、毛蕊异黄酮的含量,并比较四种不同供试液中7种成分的含量差异,实验采用Agilent 5 TC-C_(18)色谱柱(250 mm×4.6 mm,5μm),0.3%甲酸水溶液-乙腈进行梯度洗脱,流速为1.0 mL/min,检测波长254 nm;柱温30℃,漂移管温度70℃。在该色谱条件下,黄芪中7种成分能得到较好的分离,稳定性,重复性及精密度良好。本方法能简便、快捷、有效的测定黄芪药材中多种成分含量。大孔树脂制备供试品中黄芪皂苷和黄酮类成分更高,说明碱化处理对黄芪中的成分含量产生了一定影响。

HPLC-UV-ELSD method was developed for the simultaneous determination of astragaloside I,astragaloside II,astragaloside III,astragaloside IV,calycosin glucoside,ononin,and calycosin in Astragali Radix.This method was then used to determine the contents of seven active analytes in four different testing samples.The seven compounds were analyzed simultaneously with an Agilent C18(250 mm×4.6 mm,5μm)column by gradient elution using 0.3%(v/v)formic acid-acetonitrile as the mobile phase at the flow rate of 1.0 mL/min;The detection wavelength was set at 254 nm,the column temperture was maintained at 30℃,and the evaporative light-scattering detector(ELSD)drift tube temperature was 70℃.The seven active analytes of Astragali Radix can be separated in the chromatographic conditions.Precision,repeatability and stability were good.The developed method was simple,rapid and effective for the determination of the components in Astragali Radix.As a result,astragaloside and flavonoids were higher in the preparation of macroporous resin,indicating that alkalization had an effect on the content of composition in Astragali Radix.