黑土长期定位试验原状土搬迁对土壤细菌群落多样性的影响

Effects of Black Soil Long-term Test Undisturbed Soil Moved on Soil Bacterial Community Diversity

为了研究黑土长期定位试验原状土整体搬迁对土壤细菌群落结构多样性的影响,采用PCR-DGGE及其特异性条带克隆测序的方法,对同为小麦茬的搬迁前2010年和搬迁后2013年土壤细菌群落结构进行研究。结果表明:细菌DGGE图谱中搬迁前后两个土层不同处理所有样品之间具有相似性,进一步对细菌DGGE图谱进行聚类分析可得,细菌群落结构在两个土层各处理中搬迁前后变化不大。再对细菌DGGE图谱主成分分析结果可得,在0~20 cm土层细菌各处理的分布虽然搬迁前和搬迁后产生分异,但在搬迁前后两年中处理间的分布规律相似,在20~40 cm土层搬迁前和搬迁后细菌的MNPK和NPK处理分布规律发生了变化。研究说明,搬迁前和搬迁后土壤中细菌群落结构受到耕作方式、植被类型、土壤肥力等的影响远大于搬迁的扰动影响影响。

In order to study the effects of black soil long- term test undisturbed soil moved on soil bacterial community structure diversity, the soil bacterial community structure were examined of before moving 2010 and after moving 2013 as wheat stubble using PCR- DGGE and the specific bands cloning sequencing. Results showed that, all the samples of the before and after the removal had the similarities in the bacterial DGGE profiles. The cluster analysis of the DGGE profiles showed that, the changes in bacterial community structure of each treatment were not significant between the before and after the removal. The principal component analysis of the DGGE profiles showed that, in 0 ~ 20 cm soil layer, bacterial community structure of each treatment produced variation, between the before and after the removal,but the distribution law of treatments was consistent in two years, in 20 ~ 40 cm soil layer. The law of bacterial community structure of MNPK and NPK treatment changed between the before and after the removal. In summary, the disturbance influences of the removal on soil bacterial community structure were far less than the influences of farming methods, vegetation types, soil fertility and so on.