HPS对HepG_2细胞增殖的影响

Effects of HPS on the proliferation of HepG2 cells

旨在探讨红芪多糖(Hedysarum polybotrys polysacchcrides,HPS)对人肝癌细胞HepG_2增殖的影响。方法:以不同浓度HPS处理HepG_2,观察细胞形态;以700μg/ml茶多酚作为阳性对照,MTT法检测HepG_2细胞增殖情况。利用酶联免疫检测仪分别在570nm、530nm、495nm、490nm和470nm波长下测定各孔吸光值,确定最佳波长,计算抑制率,测定HPS对HepG_2的半数抑制浓度(IC50)。结果:495nm波长为测定HPS对HepG_2处理后光吸收值的最佳波长。高浓度HPS处理HepG_2,细胞形态变化明显。HepG_2增殖抑制率与HPS浓度呈正相关。HPS 9000μg/ml时,抑制率达91%.结论:MTT法检测HepG_2增殖情况的最佳波长为495nm.低浓度HPS刺激HepG_2细胞生长。浓度高于6000μg/ml时,HPS对HepG_2细胞的抑制率明显上升,最高抑制率为91%,半数抑制浓度(IC50)为6587.967μg/ml.

Objective: To investigate the effect of HPS on the proliferation of HepG2.Methods: Different concentra tions of HPS were used to observe the cell morphology of HepG2 cultured in vitro. MTT method was used to observethe proliferation of HepG2 cells with 700μg/ml of TP as positive control. Respectively at 570 nm, 530 nm, 495 nm,490 nm and 470 nm,using enzyme-linked immunoassay instrument determines the hole light absorption value, the op-timal wavelength, the inhibition rate calculated and the HPS on HepG2 cells half inhibitory concen tration(IC50).lts: The 495 nm is the best wavelength to determine the absorbance of HPS treat ed HepG2 cells. HepG2 Resu-were treated with the high concentrations of HPS, cell morphology changes significantly. The inhibi tion rate ofHepG2 cells was positively correlated with the concentration of HPS.When the concentra tion of HPS was 9000μg/ml, the inhibition rate was as high as 91%. Conclusions: The best detection wave length of HepG2 cell prolifera-tion by MTT was 495 nm. Low concentration HPS stimulat ed the growth of HepG2 cells. When the concentration was higher than 6000μg/ml, the inhibi tion rate of HepG2 cells by HPS was obviously increased, the maximum inhibition rate was 91%, and the half inhibition concentration(IC50) was 6587.967μg/ml.