混浊介质多光子激发显微成像的快速模型

Rapid Modeling of MPE Microscopic Imaging Through Turbid Medium

将二维点扩散函数 (PSF)和蒙特卡罗方法相结合 ,引入了一种研究混浊介质显微成像的快速仿真模型。将该模型用于混浊介质的多光子激发 (MPE)显微成像研究 ,极大地提高了模拟效率。与直接蒙特卡罗方法计算结果的比较证实了该方法的正确性和有效性。此外 ,一个计算实例说明了混浊介质的多光子激发显微成像具有比共焦荧光显微成像更优的横向分辨率。

A rapid and accurate model used to study microscopic imaging through turbid medium is presented which combines traditional Monte Carlo with two dimensional point spread function (PSF). The research shows that the rapid model is much faster than a pure Monte Carlo method with the help of convolution operation, when it is used to simulate multi photon excitation (MPE) fluorescence scanning microscopic imaging through turbid medium. Besides, one run instance illustrates that higher transverse resolution can be obtained in imaging through turbid medium by MPE than by confocal fluorescence microscopy.