菲律宾蛤生殖机制的研究——Ⅱ.氨海水促使卵母细胞成熟的超微结构研究

A STUDY ON THE REPRODUCTIVE MECHANISM OF RUDITAPES PHILIPPINARUM Ⅱ. ULTRASTRUCTURAL STUDY ON PROMOTING OF EGG MATURITY BY NH_4OH SEA WATER

Loosanoff(1963)提出在海水中加入氢氧化铵配成适宜浓度的氨海水溶液,可促使双壳类(如Tapes,Sedidecussata)卵母细胞生殖胚泡的破裂,有利于卵的受精。陈毓山(1966)、齐秋贞等人(1981)也进行过氨海水催产作用的研究。作者(在印刷中)在氨海水生理作用的研究中,除证实适宜浓度的氨海水确有此作用外,还进一步肯定了氨海水是菲律宾蛤卵母细胞达到生理成熟的必要因子,同时对其作用机制做了初步研究,并对卵母细胞的超微结构作了初步观察。据观察,在氨海水中卵母细胞发生了一系列形态变化:

Electron microscopic observation found obvious difference between experimental and control groups on eggs,ultrastrueture.Five minutes after treatment with NH4OH sea water,The ultrastructural change had occurred in the nucleus and cytoplasm;10 minutes later,the changes were more obvious.The nucleus moved from centre to the nuclear membrane.The number of mitoehondria increased.The Golgi complex and rough endoplasmie reticulum(RER)was highly developed.After 20—30 minutes,the metabolic activity of the eggs reached the highest level.The nueleolus in the nucleus was closely in contact with the nuclear membrane)the outer membrane of the nucleus was obviously contacted with RER.A variety of morphological mitochondria occurred in the cytoplasm)the annulate lamellae and Golgi complex was most developed.These changes indicated that the metabolic activity of the nucleus and cytoplasm was very thriving.Our electron microscopic research showed that the annulate lamellae played an important role in promoting the maturity of the egg cells.Thus,the mechanism of the action of NH4OH sea water was achieved through the stimulation of metabolic activity of egg nucleus and cytoplasm.