大黄素对抗顺铂引起的WI-38细胞凋亡

Inhibitory effect of emodin on WI-38 cell apoptosis induced by cisplatin

目的 从细胞水平研究大黄素对顺铂引起WI 38细胞凋亡的影响。方法 采用MTT法检测细胞毒性 ,用形态学观察、DNA凝胶电泳及流式细胞仪检测细胞凋亡。结果 WI 38细胞经大黄素和顺铂同时处理 2 2h后 ,30mg·L- 1大黄素可明显减轻顺铂引起的细胞毒性 ,其IC50 值由 (16± 3)mg·L- 1增加至 (34± 6 )mg·L- 1;可明显抑制顺铂引起的细胞形态学改变、核异染色质边集和DNA片段化 ,使顺铂 10和 30mg·L- 1导致的细胞凋亡率由 35 .5 6 %和33.99%降至 9.2 1%和 10 .2 5 % ,S期细胞百分数由6 2 .6 6 %和 4 8.4 6 %降至 4 8.6 7%和 36 .18%。结论 大黄素可对抗顺铂所致WI 38细胞凋亡 。

AIM To study if emodin could protect WI 38 cell against apoptosis induced by cisplatin(cis diaminedichloroplatinum). METHODS Cell inhibition rate was determined by MTT colorimetry method. Cellular morphological characteristics was observed by optical microscopy and electron microscopy. DNA fragmentation was determined by flow cytometry and agarose gel electrophoresis. RESULTS After WI 38 cell was incubated with cisplatin and emodin(30 mg·L -1 ) for 22 h, it was showed that cytotoxicity of cisplatin was reduced apparently by emodin , which was characterized by the increase in IC 50 from (16±3)mg ·L -1 to (34±6)mg·L -1 , that the changes of cellular morphology were recovered by emodin , that chromatin condensation and DNA fragmentation were remarkably inhibited by emodin, that the apoptotic rate elevated by cisplatin (10, 30 mg·L -1 ) was reduced by emodin from 35.56% and 33.99% to 9.21% and 10.25% , respectively, and that cell numbers in S phase were reduced by emodin from 62.66% and 48.46% to 48.67% and 36.18%, respectively. CONCLUSION Emodin can protect WI 38 cell against apoptosis induced by cisplatin, it might be correlated with its effect on cell cycle.